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Abs to Myosin
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Anti -MYPT1, phosphorylated (Thr696) (Myosin Phosphatase Target Subunit 1, Myosin Phosphatase-targeting Subunit 1, MBS, MGC133042, Protein Phosphatase 1 Regulatory Subunit 12A, PPP1R12A, Protein Phosphatase Myosin-binding Subunit)
Anti -MYPT1, phosphorylated (Thr696) (Myosin Phosphatase Target Subunit 1, Myosin
Phosphatase-targeting Subunit 1, MBS, MGC133042, Protein Phosphatase 1 Regulatory Subunit
12A, PPP1R12A, Protein Phosphatase Myosin-binding Subunit)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B |
|
| Protein phosphatase 1 (PP1) is a ubiquitous eukaryotic protein serine/threonine phosphatase involved in the regulation of various cell functions. Substrate specificity is determined by the binding of a regulatory subunit to the PP1 catalytic subunit (PP1c). It is estimated that over fifty different regulatory subunits exist. The myosin phosphatase holoenzyme is composed of three subunits: the PP1 catalytic subunit (PP1c), a targeting/regulatory subunit (MYPT/myosin-binding subunit of myosin phosphatase) and a 20kD subunit of unknown function (M20). MYPT binding to PP1cd alters the conformation of the catalytic cleft and increases enzyme activity and specificity. Two MYPT isoforms that are 61% identical have been described. MYPT1 is widely expressed while MYPT2 expression appears to be exclusive to heart and brain. Related family members also include MBS85, MYPT3 and TIMAP. | | | Catalog # | M9925-09C | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilutions | Western Blot: 1:1000 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | Not Determined | | Form | Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Synthetic phosphopeptide corresponding to residues surroundiing Thr696 of human MYPT1. | | Specificity | Recognizes endogenous levels of human MYPT1 at ~140kD only when phosphorylated at threonine 696. Species Crossreactivity: monkey, mouse and rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 12A |
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