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You are here:Home » Antibodies » Abs to Myosin » Anti -MYPT1, phosphorylated (Thr696) (Myosin Phosphatase Target Subunit 1, Myosin Phosphatase-targeting Subunit 1, MBS, MGC133042, Protein Phosphatase 1 Regulatory Subunit 12A, PPP1R12A, Protein Phosphatase Myosin-binding Subunit)

Anti -MYPT1, phosphorylated (Thr696) (Myosin Phosphatase Target Subunit 1, Myosin
Phosphatase-targeting Subunit 1, MBS, MGC133042, Protein Phosphatase 1 Regulatory Subunit
12A, PPP1R12A, Protein Phosphatase Myosin-binding Subunit)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Protein phosphatase 1 (PP1) is a ubiquitous eukaryotic protein serine/threonine phosphatase involved in the regulation of various cell functions. Substrate specificity is determined by the binding of a regulatory subunit to the PP1 catalytic subunit (PP1c). It is estimated that over fifty different regulatory subunits exist. The myosin phosphatase holoenzyme is composed of three subunits: the PP1 catalytic subunit (PP1c), a targeting/regulatory subunit (MYPT/myosin-binding subunit of myosin phosphatase) and a 20kD subunit of unknown function (M20). MYPT binding to PP1cd alters the conformation of the catalytic cleft and increases enzyme activity and specificity. Two MYPT isoforms that are 61% identical have been described. MYPT1 is widely expressed while MYPT2 expression appears to be exclusive to heart and brain. Related family members also include MBS85, MYPT3 and TIMAP.
Catalog #M9925-09C
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to residues surroundiing Thr696 of human MYPT1.
SpecificityRecognizes endogenous levels of human MYPT1 at ~140kD only when phosphorylated at threonine 696. Species Crossreactivity: monkey, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesProtein Phosphatase 1, Regulatory (Inhibitor) Subunit 12A


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