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You are here:Home » Antibodies » Abs to Myosin » Anti -MYPT1, MYPT2 (Myosin Phosphatase 1,2)

Anti -MYPT1, MYPT2 (Myosin Phosphatase 1,2)


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Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IC FC
The major protein phosphatase-1 (PP1) is composed of 3 subunits with apparent molecular mass of 110-130, 37 & 20kD. The 110-130kD component act as a regulatory subunit known as myosine binding (MBS) or myosine phosphatae targeting subunit (MYPT). The N-terminal portion of MYPT binds to both PP1c-delta and phosphorylated myosin, thereby increasing myosin phosphatase activity. Two isoform of MYPT have been identified (MYPT1 & MYPT2) and share 61% sequence identity. While MYPT1 is widely distributed in human tissues, MYPT2 is mostly detected in brain and heart.
Catalog #M9925-13
ApplicationsSuitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:100
Western Blot: 1:5,000
Immunocytochemistry: 1:100
Immunoprecipitation: 1:80
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
Clone No6k279
FormSupplied as a liquid.
ImmunogenA synthetic peptide corresponding to residues in C-terminus of human MYPT1 or MYPT2
SpecificityRecognizes human MYPT1 or MYPT2
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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