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You are here:Home » Antibodies » Abs to Enzymes, Protease (Proteinase, Peptidase) » Anti -NAALADase-Like 2 (N-acetylated A-linked Acidic Dipeptidase Like 2, NAALADL2)

Anti -NAALADase-Like 2 (N-acetylated A-linked Acidic Dipeptidase Like 2, NAALADL2)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IP IC FC
NAALADL2 (N-acetylated a-linked acidic dipeptidase like 2) is a member of the peptidase M28 family of enzymes. It is presumably a type II transmembrane (TM) protein that may have O-glycosyl hydrolase activity. Human NAALADL2 is 795 amino acids (aa) in length. It is believed to contain a cytoplasmic and TM segment at the N-terminus, followed by a peptidase domain (aa 444-596) and a TfR-like dimerization region (aa 688-777). There are multiple splice forms. One shows a 4 aa substitution for aa 292-795, a second shows an alternate start site at Met18 with a 24 aa substitution for aa 314-795, and a third contains an alternate start site at
human NAADADL2 is 87% and 82% aa identical to mouse and canine NAADADL2, respectively.
Catalog #N0002-45
ApplicationsSuitable for use in Flow Cytometry, Direct ELISA, Western Blot, Immunocytochemistry and Immunoprecipitation. Other applications not tested.
Recommended DilutionFlow Cytometry: use 10ul to label 10e6 cells.
Direct ELISA: 0.5 -1ug/mL detection limit for rhNAALADL2 ~ 0.3ng/well.
Western Blot: 0.1-0.2ug/ml detection limit for rhNAALADL2 ~ 10ng/lane under non-reducing and reducing conditions.
Immunocytochemistry: 10ug/mL
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from PBS with 5% trehalose. Reconstitute with 200ul 40-50% glycerol, PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenNS0-derived, recombinant human NAALADL2. (aa 152 - 795)
SpecificityRecognizes human NAALADL2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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