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You are here:Home » Antibodies » Antibodies-Antibiotics » Anti -Nalidixic Acid

Anti -Nalidixic Acid


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Clone Host Grade Applications
Polyclonal Sheep Purified E
Catalog #N0013
Inhibitor of bacterial DNA synthesis. Nalidixic acid is the first of the synthetic quinolone antibiotics. It is a naphthyridone, not a quinolone its ring structure is a 1,8-naphthyridine nucleus that contains two nitrogen atoms, unlike quinoline, which has a single nitrogen atom.[1] Synthetic quinolone antibiotics were discovered by George Lesher and coworkers as a byproduct of chloroquine manufacture in the 1960s.[1] Used clinically from 1967.[1]
Nalidixic acid is effective against both gram-positive and gram-negative bacteria. In lower concentrations, it acts in a bacteriostatic manner; that is, it inhibits growth and reproduction. In higher concentrations, it is bactericidal, meaning that it kills bacteria instead of merely inhibiting their growth.
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended Dilution:
ELISA: 0.625ug/ml
Optimal dilutions to be determined by the researcher.
Match To N0015-75
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
Concentration~1mg/ml (U.V. abs)
FormSupplied as a liquid in 20mM PBS, 0.09% sodium azide, pH 7.2.
ImmunogenNalidixic Acid-BTG.
SpecificityRecognizes Nalidixic Acid.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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