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You are here:Home » Antibodies » Abs to Proteins » Anti -NAG-1 (PTGF-b, PLAB, PDF, MIC-1)

Anti -NAG-1 (PTGF-b, PLAB, PDF, MIC-1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
NAG-1 is a TGF beta family member that is transcriptionally induced by p53. It induces growth arrest, inhibits tumorigenesis, and can be pro-apoptotic on colorectal cancer cells.
Catalog #N0017
ApplicationsSuitable for use in Immunohistochemistry and Western Blot. Other applications have not been tested.
Recommended DilutionsImmunohistochemistry: Reported to detect NAG-1 in formalin-fixed, Triton-X permeabilized, colon and lung paraffin sections. Antigen unmasking with sodium citrate required.
Western Blot: 2ug/ml detects the 35kD proform of NAG-1 from HCT-116 and LNCap cell lystaes.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb
I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa287-302, K-KTDTGVSLQTYDDLLA of human NAG-1, conjugated to Ovalbumin.
SpecificityRecognizes both pro- and secreted-forms of human NAG-1 at ~35kD and 10-15kD respectively. Does not crossreact with mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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