| NAG-1 is a TGF beta family member that is transcriptionally induced by p53. It induces growth arrest, inhibits tumorigenesis, and can be pro-apoptotic on colorectal cancer cells. |
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| Catalog # | N0017 |
| Applications | Suitable for use in Immunohistochemistry and Western Blot. Other applications have not been tested. |
| Recommended Dilutions | Immunohistochemistry: Reported to detect NAG-1 in formalin-fixed, Triton-X permeabilized, colon and lung paraffin sections. Antigen unmasking with sodium citrate required. |
| Western Blot: 2ug/ml detects the 35kD proform of NAG-1 from HCT-116 and LNCap cell lystaes. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-46Q: IgG, H&L (HRP) Pab Gt xRb |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Synthetic peptide corresponding to aa287-302, K-KTDTGVSLQTY DDLLA of human NAG-1, conjugated to Ovalbumin. |
| Specificity | Recognizes both pro- and secreted-forms of human NAG-1 at ~35kD and 10-15kD respectively. Does not crossreact with mouse. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
