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You are here:Home » Antibodies » Abs to Inflammasomes » Anti -NALP3 (NACHT-, LRR- and PYD-Containing Protein 3, AGTAVPRL, AII/AVP, Angiotensin/Vasopressin Receptor AII/AVP-like, C1orf7, Cold Autoinflammatory Syndrome 1 Protein, CIAS1, Cryopyrin, Familial Cold Autoinflammatory Syndrome, FCAS, FCU, Muckle-Wells Syndro

Anti -NALP3 (NACHT-, LRR- and PYD-Containing Protein 3, AGTAVPRL, AII/AVP,
Angiotensin/Vasopressin Receptor AII/AVP-like, C1orf7, Cold Autoinflammatory Syndrome 1
Protein, CIAS1, Cryopyrin, Familial Cold Autoinflammatory Syndrome, FCAS, FCU,
Muckle-Wells Syndro

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified B
NALP3 is a cytoplasmic 115-130kD member of the NLRP family of molecules. It is selectively expressed, being found in stratified squamous and transitional epithelium, Hassell's corpuscles, neutrophils, dendritic cells and T cells. NALP3 promotes IL-1b and IL-18 maturation by activating caspase-1. It does so by forming a NALP3 inflammasome comprised of NALP3, ASC and caspase-1. Bacterial RNA and cell wall peptidoglycan can bind to NALP3, promoting (potentially) ATP binding, NALP3 oligomerization, and caspase activation. Human NALP3 is 1036aa in length. It contains an N-terminal DAPIN domain (aa1-93), a NACHT domain (aa220-536), and seven consecutive LRRs (aa740-991). Alternate splice forms are reported. Either individually, or in combination, there can be deletions of aa836-892, 721-777, and a premature truncation after Gly719 that generates a 70kD isoform. Over aa3-223, human NALP3 shares 76aa identity with mouse NALP3.
Catalog #N0018-64J1
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.5ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 200ug/ml.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to aa4-223 from human NALP3 expressed in E. coli (Q96P20).
SpecificityRecognizes human NALP3. Species sequence homology: recombinant human NALP1 and rhNALP2 (1%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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