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You are here:Home » Antibodies » Abs to Homeobox (HOX) » Anti -Nanog (Early Embryo Specific Expression NK Family Gene, Embryonic Stem Cell Specific Homeobox Protein, ENK, FLJ12581, FLJ40451, Homeobox Transcription Factor Nanog, Nanog Homeobox)

Anti -Nanog (Early Embryo Specific Expression NK Family Gene, Embryonic Stem Cell Specific
Homeobox Protein, ENK, FLJ12581, FLJ40451, Homeobox Transcription Factor Nanog, Nanog
Homeobox)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
The transcriptional factor Nanog functions in maintaining pluripotency in cooperation with other key genes such as Oct4. Oct4 and Sox2 bind to the Nanog promoter in mouse and human embryonic stem cell. Nanog levels allow stem cells to balance the creation of lineage-committed and undifferentiated cells.
Catalog #N0019-01
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsELISA: 0.5-2ug/ml Detects 0.2-0.4ng/well of recombinant hNanog.
Western Blot: 0.1-0.2ug/ml Detects limit for recombinant hNanog is 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH7.2.
PurityPurified by immunoaffinity chromatography.
ImmunogenHighly pure (> 98%) recombinant human Nanog
SpecificityRecognizes human Nanog.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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