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Anti -Nanog (Early Embryo Specific Expression NK Family Gene, Embryonic Stem Cell Specific Homeobox Protein, ENK, FLJ12581, FLJ40451, Homeobox Transcription Factor Nanog, Nanog Homeobox) (Biotin)
Anti -Nanog (Early Embryo Specific Expression NK Family Gene, Embryonic Stem Cell Specific
Homeobox Protein, ENK, FLJ12581, FLJ40451, Homeobox Transcription Factor Nanog, Nanog
Homeobox) (Biotin)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B |
|
| The transcriptional factor Nanog functions in maintaining pluripotency in cooperation with other key genes such as Oct4. Oct4 and Sox2 bind to the Nanog promoter in mouse and human embryonic stem cell. Nanog levels allow stem cells to balance the creation of lineage-committed and undifferentiated cells. | | | Catalog # | N0019-01A | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilutions | ELISA (Direct and Sandwich): 0.25-1ug/ml. Use100ul/well antibody solution. Detects at least 0.2-04ng/well of recombinant hNanog. | | Western Blot: 0.1-0.2ug/ml. The detection limit is 1.5-3ng/lane, under either reducing or non-reducing conditions. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, before the addition of glycerol to 40%. Labeled with Biotin. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Highly pure (> 98%) recombinant human Nanog. | | Specificity | Recognizes human Nanog. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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