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You are here:Home » Antibodies » Abs to Homeobox (HOX) » Anti -Nanog (Early Embryo Specific Expression NK Family Gene, Embryonic Stem Cell Specific Homeobox Protein, ENK, FLJ12581, FLJ40451, Homeobox Transcription Factor Nanog, Nanog Homeobox)

Anti -Nanog (Early Embryo Specific Expression NK Family Gene, Embryonic Stem Cell Specific
Homeobox Protein, ENK, FLJ12581, FLJ40451, Homeobox Transcription Factor Nanog, Nanog
Homeobox)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
Nanog is a multidomain homeobox transcription factor that functions to maintain the undifferentiated state of pluripotent stem cells. Nanog expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. When Nanog expression is downregulated, cell differentiation can proceed. Proteins that regulate Nanog expression include transcription factors Oct4, SOX2, FoxD3, and Tcf3 and tumor suppressor p53.
Catalog #N0019-01R
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1:1000
Immunohistochemistry: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No10C313
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied in liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant human Nanog (1-154aa) purified from E. coli
SpecificityRecognizes human Nanog (1-154aa)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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