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You are here:Home » Antibodies » Abs to Homeobox (HOX) » Anti -Nanog (Early Embryo Specific Expression NK Family Gene, Embryonic Stem Cell Specific Homeobox Protein, ENK, FLJ12581, FLJ40451, Homeobox Transcription Factor Nanog, Nanog Homeobox)

Anti -Nanog (Early Embryo Specific Expression NK Family Gene, Embryonic Stem Cell Specific
Homeobox Protein, ENK, FLJ12581, FLJ40451, Homeobox Transcription Factor Nanog, Nanog
Homeobox)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites E B
Nanog is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human Nanog protein shares 52% overall amino acid identity with the mouse protein and 85% identity in the homeodomain. Human Nanog maps to gene locus 12p13.31, whereas mouse Nanog maps to gene loci 6 F2. Murine embryonic Nanog expression is detected in the inner cell mass of the blastocyst. High levels of human Nanog expression were detected by Northern analysis in the undifferentiated N-Tera embryonal carcinoma cell line.
Catalog #N0019-01S
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:10,000
Western Blot: 1: 500-1:2000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No10F609
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as liquid, 0.04% sodium azide.
PurityAscites
ImmunogenPurified recombinant fragment corresponding to aa20-166 of NANOG expressed in E. coli.
SpecificityRecognizes human NANOG at 35kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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