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You are here:Home » Antibodies » Abs to Transcription Factors, Homeobox (HOX) » Anti -NANOG (Homeobox Protein NANOG, Homeobox Transcription Factor Nanog, hNanog)

Anti -NANOG (Homeobox Protein NANOG, Homeobox Transcription Factor Nanog, hNanog)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
NANOG is a unique variant of NK2 family of transcription factors. It is a human ortholog of mouse Nanog and consists of a highly conserved homeodomain and Trp-rich repeats. It is specifically expressed by embryonic stem cells and is crucial for second embryonic lineage determination. It works in concert with OCT-4 and SOX-2 and plays a central role in regulating pluripotency and self-renewal of ICM and ES cells. It functions independent of LIF:gp130:Stat3 pathway and prevents differentiation of ES cells to extraembryonic endoderm and trophectoderm lineages. Its expression has also been detected in embryonic carcinoma cells and germ line tumors indicating a therapeutic intervention.
Catalog #N0019-01U
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-2ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlHeLa (human), NIH 3T3 (mouse)
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
PurityPurified by Protein G affinity Chromatography.
ImmunogenSynthetic peptide corresponding to aa1-50 of human NANOG.
SpecificityRecognizes human NANOG. Species Crossreactivity: chimpanzee, goat, mouse, rhesus monkey.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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