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Anti -NANOG (Homeobox Transcription Factor Nanog, Homeobox Protein NANOG, hNanog)
Anti -NANOG (Homeobox Transcription Factor Nanog, Homeobox Protein NANOG, hNanog)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E B IH |
|
| NANOG is a Ttranscription regulator involved in inner cell mass and embryonic stem (ES) cels proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation. | | | Catalog # | N0019-01U2 | | Applications | Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | ELISA: 1:128,000 | | Western Blot: 0.05-0.1ug/ml, observed in nuclear lysates of cell line HeLa on ~35kD bands | | Immunohistochemistry (Paraffin): 1-2ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Goat | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide corresponding to C-DLFLNYSMNMQPED, from human NANOG, at C+terminal (NP_079141.2). | | Specificity | Recognizes human NANOG. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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