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You are here:Home » Antibodies » Abs to Peptides » Anti -Natriuretic Peptide, C-Type (CNP)

Anti -Natriuretic Peptide, C-Type (CNP)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Purified E B
C-Type Natriuretic Peptide (CNP) belongs to the natriuretic peptide family and functions in an autocrine or paracrine fashion. CNP interacts with the GC-B/NPR-B receptor to promote vasorelaxation, vascular remodeling, and the growth and differentiation of bone and neural tissue. CNP is synthesized as a prohormone that is cleaved intracellularly by furin, yielding a 49aa propeptide and a 53aa mature peptide. Additional cleavage of the mature peptide generates 29aa and 22aa peptides which have comparable activity in some assays. Within the 53aa mature CNP, human shares 96% aa sequence identity with mouse and rat.
Catalog #N0019-05J
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionDirect ELISA: 0.5-1.0ug/ml. The detection limit is ~20ng/well.
Western Blot: 1-2ug/ml detects human CNP peptides containing aa98-126. Using a colormetric system, the detection limit is ~25ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
IsotypeIgG2b
Clone No8H204
HostRat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenE. coli-derived recombinant human CNP precursor. (aa24-126, Accession #P23582)
SpecificityRecognizes human CNP (aa98-126) and human CNP (aa24-126). Does not crossreact with recombinant human ANP or BNP. Species sequence homology: mouse and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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