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Specifications

Clone Host Grade Applications Monoclonal Rat Purified E B

C-Type Natriuretic Peptide (CNP) belongs to the natriuretic peptide family and functions in an autocrine or paracrine fashion. CNP interacts with the GC-B/NPR-B receptor to promote vasorelaxation, vascular remodeling, and the growth and differentiation of bone and neural tissue. CNP is synthesized as a prohormone that is cleaved intracellularly by furin, yielding a 49aa propeptide and a 53aa mature peptide. Additional cleavage of the mature peptide generates 29aa and 22aa peptides which have comparable activity in some assays. Within the 53aa mature CNP, human shares 96% aa sequence identity with mouse and rat.
Catalog #	N0019-05J
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~20ng/well.
	Western Blot: 1-2ug/ml detects human CNP peptides containing aa98-126. Using a colormetric system, the detection limit is ~25ng/lane under non-reducing and reducing conditions.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2b
Clone No	8H204
Host	Rat
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity	Purified by Protein G affinity chromatography.
Immunogen	E. coli-derived recombinant human CNP precursor. (aa24-126, Accession #P23582)
Specificity	Recognizes human CNP (aa98-126) and human CNP (aa24-126). Does not crossreact with recombinant human ANP or BNP. Species sequence homology: mouse and rat
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.