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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified E B

Nanos2 is a cytoplasmic RNA-binding protein that belongs to the Nanos family of translational repressors. The 138 amino acid (aa) protein contains a 55 aa nanos-type zinc finger domain with two C2HC motifs. Human and mouse Nanos2 share identity 75% aa sequence identity overall but 95% within the zinc finger RNA binding domain. In the mouse, Nanos2 is located in male germ cells and is essential for spermatogenesis.
Catalog #	N0019-40
Applications	Suitable for use in Direct ELISA and Western Blot. Other applications not tested.
Recommended Dilution	Direct ELISA: 0.5-1.0ug/ml detection limit for rhNanos2 is ~ 2.5ng/well.
	Western Blot: 1-2ug/ml detection limit for rhNanos2 is ~ 5ng/lane under non-reducing and reducing conditions.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	8H151
Host	Mouse
Source	Human
Concentration	As reported
Form	Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile 40-50% glycerol, PBS.
Purity	Purified by Protein G affinity chromatography.
Immunogen	E. coli-derived rhNanos2 (aa 1 - 173)
Specificity	Recognizes human Nanos2.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.