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You are here:Home » Antibodies » Antibodies-Ion Transport » Anti -Natural Resistance Associated Macrophage Protein (Nramp2)

Anti -Natural Resistance Associated Macrophage Protein (Nramp2)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Natural resistance to infection with unrelated intracellular parasite such as Mycobacteria, Salmonella, and Leishmania is controlled by a single gene that encodes a macrophage-specific membrane protein designated as Natural Resistance-Associated Macrophage Protein (Nramp1). Recently a second member of NRAMP family, termed NRAMP2/DMT/DCT1 (Divalent Metal ion Transporter 1 or Divalent Cation Transporter 1), has been identified (human, rat and mouse 568 aa, ~65% identity with NRAMP1).
Catalog #N0020-01A
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1-10ug/ml using ECL technique
ELISA: 0.5-1ug/ml Use 1ug/ml control peptide/well
Immunohistochemistry: 5-20ug/ml. Use paraformaldehyde fixed sections.
Optimal dilutions to be determined by the researcher.
Recommended Control PeptideN0020-01: Natural Resistance Associated Macrophage Protein, Rat (Nramp2)
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, 0.1% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenA 19 aa synthetic peptide sequence located at the predicted cytoplasmic loop #4 of rat NRAMP2 was synthesized, coupled to KLH. Species sequence homology: 100% mouse, 94% human, 73% zebra fish NRAMP2.
SpecificityRecognizes rat Natural Resistance Associated Macrophage Protein (Nramp2). This sequence is common in NRAMP2 with or without IRE isoforms. This antibody will not distinguish between these isoforms.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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