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You are here:Home » Antibodies » Abs to Ion Transport » Anti -Natural Resistance Associated Macrophage Protein (Nramp2)

Anti -Natural Resistance Associated Macrophage Protein (Nramp2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Natural resistance to infection with unrelated intracellular parasite such as Mycobacteria, Salmonella, and Leishmania is controlled by a single gene that encodes a macrophage-specific membrane protein designated as Natural Resistance-Associated Macrophage Protein (Nramp1). Recently a second member of NRAMP family, termed NRAMP2/DMT/DCT1 (Divalent Metal ion Transporter 1 or Divalent Cation Transporter 1), has been identified (human, rat and mouse 568 aa, ~65% identity with NRAMP1). Unlike NRAMP1, NRAMP2 expression is more ubiquitous and has been detected in most tissues. It is dramatically up-regulated by iron starvation in the intestine. NRAMP2 gene produces two alternatively spliced transcripts generated by alternative use of two 3' exons encoding distinct C-termini of the protein as well as distinct 3' untranslated regions (UTRs). Interestingly, one Nramp2 mRNA contains an iron-responsive element (IRE) in its 3'UTR. The IRE is an RNA secondary structure present in the 5'- or the 3'-UTR of animal mRNAs encoding proteins involved in iron metabolism. The second Nramp2 splice isoform (without-IRE, isoform II) encodes a protein in which the C-terminal 18-aa of the IRE form (with IRE, isoform I) are replaced by a novel 25-aa segment and codes for a distinct 3' UTR lacking the IRE. The two isoforms are differentially localized and regulated in GI tract and kidney. It has recently been demonstrated that the Nramp2 gene is mutated (Gly185 to Arg at TM4) in both the mk and Belgarde (b) animal models exhibiting a severe microcytic hypochromic anemia marked by a defect in iron absorption by intestinal cells and in erythroid iron use.
Catalog #N0020-01F
ApplicationsSuitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-10ug/ml using ECL.
ELISA: 0.5-1ug/ml. Control peptide (Cat #N0020-01H) can be used to coat ELISA plates at 1ug/ml.
Immunohistochemistry: 5-20ug/ml.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a lyophillized powder in PBS, pH 7.4, 0.1% BSA. Reconstitute with 100ul sterile PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenA 17 AA Peptide sequence located at the predicted cytoplasmic loop near the C-terminus of human NRAMP2, with IRE (1). Coupled to KLH.
SpecificityRecognizes human Natural Resistance Associated Macrophage Protein (Nramp2). Species sequence homology: monkey 88%.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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