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Anti -Natural Resistance Associated Macrophage Protein with IRE (Nramp2 with IRE)
Anti -Natural Resistance Associated Macrophage Protein with IRE (Nramp2 with IRE)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Serum |
E B |
|
| Natural resistance to infection with unrelated intracellular parasite such as Mycobacteria, Salmonella and Leishmania is controlled by a single gene that encodes a macrophage-specific membrane protein designated as Natural Resistance-Associated Macrophage Protein (Nramp1). Recently a second member of NRAMP family, termed NRAMP2/DMT/DCT1 (Divalent Metal ion Transporter 1 or Divalent Cation Transporter 1), has been identified (human, rat and mouse 568aa, ~65% identity with NRAMP1). Unlike NRAMP1, NRAMP2 expression is more ubiquitous and has been detected in most tissues. It is dramatically up-regulated by iron starvation in the intestine. NRAMP2 (slc11a2) gene produces two alternatively spliced transcripts generated by alternative use of two 3' exons encoding distinct C-termini of the protein as well as distinct 3' untranslated regions (UTRs). Interestingly, one Nramp2 mRNA contains an iron-responsive element (IRE) in its 3'UTR. The IRE is an RNA secondary structure present in the 5'- or the 3'- UTR of animal mRNAs encoding proteins involved in iron metabolism. The second Nramp2 splice isoform (without-IRE, isoform II) encodes a protein in which the C-terminal 18-aa of the IRE form (with IRE, isoform I) are replaced by a novel 25-aa segment and codes for a distinct 3' UTR lacking the IRE. The two isoforms are differentially localized and regulated in GI tract and kidney. | | | Catalog # | N0020-01J | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilutions | ELISA: 1:10,000-1:50,000. Coat ELISA plates at 1ug/ml with Control Peptide. See catalog #N0020-02A: Natural Resistance Associated Macrophage Protein with IRE, Rat, Control Pepetide. | | Western Blot: 1:1000-1:5000 (ECL). | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Rat | | Concentration | Not determined | | Form | Supplied as a liquid in PBS, 0.05% sodium azide. | | Purity | Serum | | Immunogen | Synthetic peptide, 17aa located near the C-terminal, cytoplasmic domain of rat NRAMP2 conjugated to KLH. Sequence is 100% homologous with mouse Nramp2 with IRE and is absent in NRAMP2 without IRE. No significant sequence homology exists with NRAMP1. | | Specificity | Recognizes rat NRAMP2 with IRE. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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