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You are here:Home » Antibodies » Abs to Enzymes, Phospholipase » Anti -NAPE-PLD (N-acyl Phosphatidylethanolamine Phospholipase D, N-Acylphosphatidylethanolamine-Hydrolyzing Phospholipase D, NAPE-hydrolyzing Phospholipase D, NAPEPLD, C7orf18, FMP30, Mbldc1)

Anti -NAPE-PLD (N-acyl Phosphatidylethanolamine Phospholipase D,
N-Acylphosphatidylethanolamine-Hydrolyzing Phospholipase D, NAPE-hydrolyzing Phospholipase
D, NAPEPLD, C7orf18, FMP30, Mbldc1)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IH IC IF
N-Acylphosphatidylethanolamine (NAPE)-hydrolyzing phospholipase D (NAPE-PLD) is a membrane-bound enzyme which releases the endocannabinoid anandamide and other bioactive N-acylethanolamines from their corresponding NAPEs in animal tissues. NAPE-PLD has been shown to be expressed by specific populations of neurons in the brain and targeted to axonal processes. Recent studies have suggested that NAEs generated by NAPE-PLD in axons may act as anterograde synaptic signaling molecules that regulate the activity of postsynaptic neurons. NAPE-PLD has also been shown to catalyze the hydrolysis of NAPE to generate OEA (oleoylethanolamide), a natural lipid amide that inhibits food intake in free-feeding rodents by prolonging latency to feed and postmeal interval.
Catalog #N0040-01A
ApplicationsSuitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionImmunofluorescence: 1:1000-1:5000
Western Blot: 1:2500-1:5000
Immunohistochemistry: 1:1000-1:5000
Immunocytochemistry: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.
Positive Controls
Mouse Brain total protein
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 3 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceMouse
ConcentrationNot Determined.
FormSupplied as a liquid in PBS, 0.1% sodium azide.
PuritySerum
ImmunogenSynthetic peptide corresponding to an internal portion of the mouse protein (within residues 1-100). [Swiss-Prot# Q8BH82].
SpecificityRecognizes mouse NAPE-PLD at ~50kD. Species Crossreactivity: Human and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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