| Human brain natriuretic peptide (BNP) is a secreted protein which is a member of the natriuretic peptide family. BNP is a cardiac hormone, which is synthesized as a pro-hormone (proBNP), and is proteolytically cleaved to release a biologically active fragment (BNP), and an inactive fragment (NT-proBNP) into the circulation. |
|
| BNP is predominantly secreted from the cardiac ventricles in response to volume and pressure overload, and results in a number of biological activities including natriuresis, diuresis, vasorelaxation, and inhibition of the sympathetic nervous system. A high concentration of BNP in the bloodstream is indicative of heart failure. |
|
| Catalog # | N0520-25 |
| Applications | Suitable for use in RIA, ELISA, Immunohistochemistry, Immunocytochemistry and Indirect Immunofluorescence. Other applications have not been tested. |
| Recommended Dilution | Immunohistochemistry: 1:1000. Stains rat brain sections using 3% paraformaldehyde and indirect immunofluorescent staining. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, 10mg/ml BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | Not determined. |
| Form | Supplied as a lyophilized powder. Reconstitute with 100ul PBS, 10mg/ml BSA. |
| Purity | Neat serum |
| Immunogen | Synthetic human brain natriuretic polypeptide covalently attached to a carrier protein. |
| Specificity | Recognizes human BNP (100%). Does not crossreact with Human Atrial Natriuretic Peptide, ACTH (human, 1-39), human Calcitonin, and Vasoactive Intestinal Peptide. Species crossreactivity: Rat. |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
