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You are here:Home » Antibodies » Abs to Ion Transport » Anti -NBC1 (Na, HCO3 Cotransporter 1, pNBC)

Anti -NBC1 (Na, HCO3 Cotransporter 1, pNBC)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Bicarbonate, along with CO2, is the major pH buffer of biological fluids. The inter-conversion of CO2 to HCO3- is mediated by family of carbonic anhydrases. Several organs specialize in the mobilization of HCO3-. The pancreatic ducts move CO2 and HCO3- from the blood into pancreatic secretions. The kidneys reabsorb massive amount of HCO3- daily (amount of NaHCO3 contained in 1 Ib of baking soda) through the glomerular flirtation of ~180 liters of plasma. To prevent a massive acidosis, the proximal tubules (PT) cells in the nephron subsequently reabsorb virtually all of HCO3-, as well as most of the filtered Na+, Cl-, and H2O. A great majority of HCO3- reabsorption occurs via trans-cellular coupling of the luminal Na-H+ exchanger 3 and Na+-H+ ATPase with the basolateral Na+ HCO3- cotransporters (NBC). Several related proteins constitute the emerging NBC family (NBC1-3) of membrane cotransporters that are found in a variety of epithelial and non-epithelial tissues, and may be tissue specific. Physiologically, NBC is electrogenic, Na+ and HCO3- dependent, Cl- independent, and inhibited by stilbenes (DIDS and SITS). The NBC family of proteins are 30-35% related to anion exchangers (AE2 and AE3; SLC4A1-SLC4A3) and display the same protein topology: (a) At least 10 TM domains with both the N and C-termini predicted to be intracellular, (b) presence of a large, glycosylated, extracellular loop between TM5 and TM6; and (c) the lysine residues are conserved at predicted DIDS-reactive sites.
Catalog #N0536-07
NBC1 (human, rat, mouse 1035 aa, also called NBC-1A/1B, hkNBC, rkNBC, pNBC, hhNBC, SLC4A4) was initially cloned from human Kidney. NBC1 is 30-35% identical with AEs. It is strongly expressed in the kidney and pancreas. The rat kidney NBC1 (rkNBC1; 1035 aa) is 86% identical to hkNBC1. Pancreatic NBC (pNBC/hhNBC/SLC4A5, human 1079 aa) has a unique n-terminal 85 aa sequence replacing the 41-aa in kNBC. pNBC is also more widely expressed (pancreas, thyroid, heart, and brain).
ApplicationsSuitable for use in ELISA, Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-10ug/ml using Chemiluminescence technique. We recommend the use of control peptide in antibody blocking experiments to establish antibody specificity. Control peptides, because of their small size (< 2kD), are not suitable for Western.
ELISA: 1:5000-1:50,000 (Coat with 50-100ng of control peptide/well).
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenA 20-aa peptide sequence within the cytoplasmic, C-terminus of rat NBC1 (1) was used to generate anti-NBC1. The peptide was coupled with KLH.
SpecificityRat NBC1 sequence is 100% conserved in rat, 85% in human and 80% in rabbit and frog NBC1/PSLC4A4/kNBC. This region is also shared 100% with mouse pNBC/PSLC4A5. Therefore, antibodies will recognize both the kidney and pancreatic/heart isoforms. No significant sequence homology of NBC1 was found with NBC2 or NBC3. Antibody crossreactivity in various species is not established.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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