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You are here:Home » Antibodies » Abs to Ion Transport » Anti -NBC3 (HCO3 Cotransporters 3)

Anti -NBC3 (HCO3 Cotransporters 3)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Bicarbonate, along with CO2, is the major pH buffer of biological fluids. The inter-conversion of CO2 to HCO3- is mediated by family of carbonic anhydrases. Several organs specialize in the mobilization of HCO3-. The pancreatic ducts move CO2 and HCO3- from the blood into pancreatic secretions. The kidneys reabsorb massive amount of HCO3- daily (amount of NaHCO3 contained in 1 Ib of baking soda) through the glomerular flirtation of ~180 liters of plasma. To prevent a massive acidosis, the proximal tubules (PT) cells in the nephron subsequently reabsorb virtually all of HCO3-, as well as most of the filtered Na+, Cl-, and H2O. A great majority of HCO3- reabsorption occurs via trans-cellular coupling of the luminal Na-H+ exchanger 3 and Na+-H+ ATPase with the basolateral Na+ HCO3- cotransporters (NBC). NBC3 (SLC4A); mouse (1089 aa, human 1044 aa) has 3 three transcripts, which are expressed in a tissue specific manner (9 kb in brain, placenta, lung, liver, muscle, kidney, pancreas, stomach, thyroid, and adrenal etc). NBC3 has two variants. NBC3 is 56% identical with NBC1 and 76% with NBC2.
Catalog #N0538-08
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-10ug/ml (ECL).
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.
Control PeptideN0538-10: NBC3, Human (HCO3 Cotransporters 3)
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.
PurityPurified by affinity chromatography.
ImmunogenA 19-aa peptide sequence within the cytoplasmic, C-terminus of human NBC3. (KLH).
SpecificityRecognizes human NBC3 (HCO3 Cotransporters 3). Species sequence homology: Chimp, 100%; rat, 83%; and Xenopus 68%.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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