| Neuronal precursor cell-expressed developmentally downregulated 4 (Nedd4) is an ~120kD E3 ubiquitin ligase. Nedd4 is involved in regulation of ubitiquin-targeted degradation of proteins. Nedd4 interacts with many different E2 enzymes, including human Ubc4, UbcH5B, UbcH5C, UbcH6 and UbcH7, suggesting it plays a role in multiple E2 ubiquitination pathways. Nedd4 contains a C2 domain and several WW domains, which recognize membrane surfaces and Pro-Tyr motifs, respectively. Nedd4 polyubiquitinates the epithelial sodium channel, and mutations that disrupt association between Nedd4 and the channel lead to Liddle’s syndrome, a hyperaldosteronism-like, hypertension disease. |
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| Catalog # | N0577-15 |
| Applications | Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested. |
| Recommended Dilutions | Western Blot: 1:10,000 detects Nedd4, WW2 domain on 10ug of mouse kidney cytosol. Mouse kidney cytosol lysate was resolved by electrophoresis, transferred to PVDF and probed with N0577-15. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. |
| Immunohistochemistry: Reported to detect Nedd4 in mouse kidney, lung and distal colon sections. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb |
| I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb |
| I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb |
| Positive Control | Kidney cytoplasmic extracts |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Rat |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | GST fusion protein corresponding to aa395-462 of the WW2 domain of rat Nedd4. |
| Specificity | Recognizes rat Nedd4, Mr ~115kD. Degradation products were also detected at Mr 55 and 60kD. Species Crossreactivity: mouse and human. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
