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Abs to Ubiquitin Ligases
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Anti -NEDD4 (E3 Ubiquitin-protein Ligase NEDD4, Cell Proliferation-inducing Gene 53 Protein, Neural Precursor Cell Expressed Developmentally Down-regulated Protein 4, NEDD-4, KIAA0093, NEDD4-1, PIG53)
Anti -NEDD4 (E3 Ubiquitin-protein Ligase NEDD4, Cell Proliferation-inducing Gene 53
Protein, Neural Precursor Cell Expressed Developmentally Down-regulated Protein 4, NEDD-4,
KIAA0093, NEDD4-1, PIG53)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Sheep |
Affinity Purified |
B |
|
| NEDD4 (Neural precursor cell-expressed developmentally down-regulated gene 4-1; also RPF1) is a 115-120kD member of the HECT E3 ubiquitin ligase family of proteins. The NEDD4 gene is widely expressed, and serves as a complement to a related gene product termed NEDD4-2/NEDD4L. Both molecules ubiquitinate multiple proteins. NEDD4-1 acts preferentially on PTEN, tumor suppressors and endocytic proteins, while NEDD4-2 acts preferentially on membrane transporters. Human NEDD4-1 is 900aa in length and contains one PKC region (aa20-124), four WW domains (aa196-505) and one E3 HECT domain (aa543-897). Caspase cleavage after Asp197 generates a 95kD C-terminal, and smaller but variable sized N-terminal fragment. There are multiple splice forms. Two show a 516aa substitution for aa1-169 and 1-97, respectively, while a third shows the latter substitution coupled to a deletion of aa170-185. Over aa211-349, human NEDD4-1 shares 83% and 72aa identity to mouse and rat NEDD4-1 and less than 25aa identity with human NEDD4-2. | | | Catalog # | N0577-15A | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 25ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Sheep | | Source | Human | | Concentration | As reported | | Form | Supplied as a lyophilized powder from a 0.2um-filtered solution in PBS, 5% trehalose. Reonstitute with 100ul PBS, 0.02% sodium azide. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | E. coli-derived, recombinant human NEDD4 (aa211-349; Accession # P46934). | | Specificity | Recognizes human NEDD4. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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