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Specifications

Clone Host Grade Applications Polyclonal Rabbit Highly Purified E B

Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs)
function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg12, Hub1. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not proteolytically processed or conjugated to other proteins. Rub1 and the corresponding human homolog Nedd8 are activated by the E1 ubiquitin-activating enzyme UBA2, that forms isopeptide linkages between thio esters. Nedd8 shows 80% homology to ubiquitin. The best known targets of Rub1 modification are members of the cullin family. Cullins are subunits of an
E3-ubiquitin ligase complex called the Skp1-/Cul1/Cdc53-F-box (SCF). The SCF promotes transfer of ubiquitin from a ubiquitin conjugating enzyme (E2) to the target protein. Rub1 modification may regulate SCF function or localization.
Catalog #	N0580-01F
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	ELISA: 1:1000-1:5000
	Western Blot: 1:500 (6kD)
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	~1mg/ml
Form	Supplied as a lyophilized powder in PBS, pH 7.2, 0.1% sodium azide. Reconstitution: Reconstitute with 500ul sterile, distilled, deionized water or sterile 40-50% glycerol for long term storage. No stabilizing proteins added.
Purity	Purified by delipidation, salt fractionation and ion exchange chromatography.
Immunogen	Full-length recombinant yeast Rub1 protein.
Specificity	Assay by immunoelectrophoresis resulted in a single precipitinarc against anti-Rabbit Serum.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.