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You are here:Home » Antibodies » Abs to Ubiquitin » Anti -Nedd8 (Neural Precursor Cell Expressed Developmentally Down-regulated 8, NEDD-8, MGC104393, MGC125896, MGC125897, NED8, Neddylin, Neural Precursor Cell Expressed Developmentally Down-regulated Gene 8, Rub1, Ubiquitin-like Protein Nedd8, Ubiquitin-like Pro

Anti -Nedd8 (Neural Precursor Cell Expressed Developmentally Down-regulated 8, NEDD-8,
MGC104393, MGC125896, MGC125897, NED8, Neddylin, Neural Precursor Cell Expressed
Developmentally Down-regulated Gene 8, Rub1, Ubiquitin-like Protein Nedd8, Ubiquitin-like
Pro

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH IC
NEDD8 (Neural precursor cell-Expressed Developmentally Down-regulated genes) is an ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis (1). NEDD8 has been shown to conjugate to nuclear proteins in a manner analogous to ubiquination and sentrinization. The conjugation process is though to be catalyzed by thee enzymes; E1 (NEDD8-activation), E2 (NEDD8-conjugation) and E3 (NEDD8-ligation). NEDD8 is known to conjugate to Cul-4A and other cullins proteins via the carboxy-terminal Gly residue for ubiquitinylation (3) while NUB1 will negatively regulate its expression (4).
Catalog #N0580-02D
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1:1000
Immunohistochemistry: 1:100-1:250
Immunocytochemistry: 1:100-1:250
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No9E393
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
PuritySupernatant
ImmunogenA synthetic peptide corresponding to residues in the N-term of human NEDD8.
SpecificityRecognizes human NEDD8 bound to cullins proteins (neddylated cullins) as well as free NEDD8.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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