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You are here:Home » Antibodies » Abs to Ubiquitin » Anti -NEDD8, NT (Ubiquitin-like Protein NEDD8, Neddylin, Neural Precursor Cell Expressed Developmentally Down-regulated Protein 8, NEDD-8)

Anti -NEDD8, NT (Ubiquitin-like Protein NEDD8, Neddylin, Neural Precursor Cell Expressed
Developmentally Down-regulated Protein 8, NEDD-8)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
NEDD8 is a ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
Catalog #N0580-02F
ApplicationsSuitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide selected from the N-terminal region of human NEDD8 (KLH).
SpecificityRecognizes human NEDD8.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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