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You are here:Home » Antibodies » Abs to Ubiquitin » Anti -Nedd8 (Neural Precursor Cell Expressed Developmentally Down-regulated 8, NEDD-8, MGC104393, MGC125896, MGC125897, NED8, Neddylin, Neural Precursor Cell Expressed Developmentally Down-regulated Gene 8, Rub1, Ubiquitin-like Protein Nedd8, Ubiquitin-like Pro

Anti -Nedd8 (Neural Precursor Cell Expressed Developmentally Down-regulated 8, NEDD-8,
MGC104393, MGC125896, MGC125897, NED8, Neddylin, Neural Precursor Cell Expressed
Developmentally Down-regulated Gene 8, Rub1, Ubiquitin-like Protein Nedd8, Ubiquitin-like
Pro

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B
NEDD8 (Neural precursor cell-expressed developmentally down-regulated gene 8; also Rub1) is a 6-8kD member of the ubiquitin family of proteins. It is expressed in striated muscle, and via its C-terminal Gly, forms covalent bonds with the cullin family of proteins. This conjugation generates ubiquitin ligase activity that regulates cell cycle proteins. Human proNEDD8 is 81aa in length and contains one ubiquitin-like domain (aa1-65), and a 4-5aa C-terminal propeptide that is cleaved to expose a C-terminal Gly residue that is used to generate a Gly-Lys intermolecular bond. Mature human NEDD8 shows 100% aa identity to NEDD8 from both mouse and rat.
Catalog #N0580-02H
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: .25ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No11D51
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute with sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant corresponding to aa2-77 from human NEDD8 expressed in E. coli (Q15843).
SpecificityRecognizes human, mouse and rat NEDD8.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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