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You are here:Home » Antibodies » Abs to Ubiquitin » Anti -NEDD9 (Neural Precursor Cell Expressed Developmentally Down-regulated protein 9, Enhancer of Filamentation 1, hEF1, CRK-associated Substrate-related Protein, CAS-L, CasL, Cas Scaffolding Protein Family Member 2, NEDD-9, Renal Carcinoma Antigen NY-REN-12,

Anti -NEDD9 (Neural Precursor Cell Expressed Developmentally Down-regulated protein 9,
Enhancer of Filamentation 1, hEF1, CRK-associated Substrate-related Protein, CAS-L, CasL,
Cas Scaffolding Protein Family Member 2, NEDD-9, Renal Carcinoma Antigen NY-REN-12,

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B
NEDD9 (also HEF1 and CASL) is a 93kD (predicted) member of the CAS family of proteins. It is widely expressed, and serves to integrate integrin b1 and BCR signaling with cell mitotic pathways. Human NEDD9 is 834aa in length. It contains one SH3 domain (aa3-65), a Tyr-rich SH2-binding region (aa90-350), one Ser-rich four a-helix bundle protein interaction site (aa350-650), and an HLH motif that mediates Cas protein dimerization (aa710-760). NEDD9 usually runs at 105-115kD in SDS-PAGE due to phosphorylation. It is also cleaved near Leu361 to generate an active N-terminal 55kD fragment. One isoform shows a deletion of aa155-188, while two others show 20-7aa substitutions for aa155-834, 1-153, respectively. Over aa153-366, human and mouse NEDD9 share 85aa identity.
Catalog #N0580-09A1
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2A
Clone No11n135
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
PurityPurified by Protein A/G affinity chromatography.
ImmunogenRecombinant corresponding to aa153-366 from recombinant human NEDD9 expressed in E. coli. (Q14511).
SpecificityRecognizes human NEDD9. Does not react with recombinant human NEDD4, rhNEDD8.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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