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You are here:Home » Antibodies » Abs to Netrin » Anti -Netrin G1 Ligand Precursor (NGL-1, LRRC4C, leucine rich repeat containing 4C KIAA1580, UNQ292, PRO331) (Biotin)

Anti -Netrin G1 Ligand Precursor (NGL-1, LRRC4C, leucine rich repeat containing 4C
KIAA1580, UNQ292, PRO331) (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified B
The Netrin-G1 ligand (NGL-1) promotes the outgrowth of thalamocortical axons. Human netrin G1 ligand is a transmembrane protein containing leucine-rich repeat (LRR) and immunoglobulin (Ig) domains that specifically interacts with netrin-G1 through its LRR region. Postsynaptic netrin-G ligand (NGL) CAMs associate with netrin-G CAMs in an isoform-specific manner and, through their cytosolic tail, with the abundant postsynaptic scaffold postsynaptic density-95 (PSD-95).
Catalog #N1193-10M
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWesterm Blot: 0.1-0.2ug/ml with the appropriate secondary reagents to detect human NGL-1. The detection limit for rhNGL-1 is approximately 2ng/lane under non-reducing and reducing
conditions. Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
Concentration~50ug/ml
FormSupplied as lyophilized from a 0.2um sterile-filtered solution in PBS, 50ug BSA. Reconstitute with sterile Tris-buffered saline pH 7.3 (20mM Tris base,150mM NaCl), 0.1% BSA. If 1ml of buffer is used, the antibody concentration will be 50ug/ml.
PurityPurified by immunoaffinity chromatography.
ImmunogenPurified, NS0-derived, recombinant human NGL-1 extracellular domain (rhNGL-1)
SpecificityRecognizes human NGL-1, extracellular domain.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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