| Mouse Netrin G2a is a member of a GPI-linked Netrin subfamily that is distantly related to classical Netrins. Netrin G2a is approximately 485aa in length. It contains an N-terminal laminin-related region plus a heparin-binding C-terminus that ends in a GPI-linkage. The laminin-related region shows a globular type VI domain followed by three variable-length EGF-like domains. Alternate splicing generates two larger isoforms. Amino acid insertions of 59aa and 34aa between EGF-like motifs 1 and 2 generate Netrin G2b and Netrin G2c, respectively. Mature mouse Netrin G2a shares 96% and 99% amino acid identity with human Netrin G2 and the equivalent region in rat Netrin G2b, respectively. |
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| Catalog # | N1193-15 |
| Applications | Suitable for use in Direct ELISA, Western Blot and Immunohistochemistry. Other applications not tested. |
| Recommended Dilutions | Direct ELISA: 0.5-1ug/ml; The detection limit for recombinant mouse Netrin G2a is ~3ng/well. |
| Western Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit for recombinant mouse Netrin-G2a is ~10ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. |
| Immunohistochemistry: 25ug/ml detects Netrin G2a in frozen normal mouse brain tissue sections. |
| Optimal dilutions to be determined by the researcher. |
| Hybridoma | Mouse myeloma with B cells obtained from a rat immunized with purified, NS0-derived, recombinant mouse Netrin G2a. |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2a |
| Clone No | 7H116 |
| Host | Rat |
| Source | Mouse |
| Concentration | ~0.5mg/ml (after reconstitution) |
| Form | Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Recombinant protein corresponding to aa18-504 from mouse Netrin G2a expressed in NSO cells (NP_598007). |
| Specificity | Recognizes mouse Netrin G2a in direct ELISAs and Western Blots. Does not crossreact with recombinant mouse Netrin G1a, Netrin 1 or Netrin 4. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
