| Murine netrin-1 precursor is a 67,768 dalton protein (604 amino acids) expressed by subpopulations of cells within the developing CNS, as well as in various non-neural tissues, including the gut, liver, heart, and prostate. In the embryonic CNS, netrin-1 is expressed by cells of the floor plate, and acts to attract axons of commissural neurons from the dorsal horn gray matter. Netrin-1 is also believed to play a role in the growth of motor axons in the PNS. |
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| Catalog # | N1193-16 |
| Applications | Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:250-1:500 |
| Immunohistochemistry: 1:100-1:200 |
| Immunocytochemistry: 1:100-1:200 |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1906 IgY, Chicken Pab Rb x Ch I1906-10 IgY, Chicken (HRP) Rb x Ch I1906-12 IgY, Chicken (FITC) Rb x Ch I1906-14 IgY, Chicken (AP) Rb x Ch |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgY |
| Host | Chicken |
| Source | Human |
| Concentration | ~0.1mg/ml |
| Form | Supplied as a liquid in PBS, 0.02% sodium azide. |
| Purity | Purified by affinity chromatography. |
| Immunogen | Peptide corresponded to a region of the Netrin 1 gene product shared between the mouse (O09118, NCBI) and human (O95631, NCBI) gene products. |
| Specificity | Recognizes human Netrin-1. Species crossreactivity: mouse. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
