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You are here:Home » Antibodies » Abs to Nephrin » Anti -Nephrin (NPHN, CNF, Nephrosis 1 Congenital Finnish Type, NPHS1, Renal Glomerulus-specific Cell Adhesion Receptor)

Anti -Nephrin (NPHN, CNF, Nephrosis 1 Congenital Finnish Type, NPHS1, Renal
Glomerulus-specific Cell Adhesion Receptor)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B IH IC
Nephrin, also known as renal glomerulus-specific cell adhesion receptor and nephrosis 1 (NPHS1) is a 185-200 kD type I transmembrane protein belonging to the immunoglobulin (Ig) superfamily. It is expressed on podocytes and is an essential component of the interpodocyte-spanning slit diaphragm complex. Nephrin forms cis-hetero-oligomeric complexes with Neph1, followed by trans-homophilic interaction with Nephrin on opposing cells. Mutations in the Nephrin gene is the pathogenic cause of congenital nephrotic syndrome. Mature human Nephrin contains a 1033 aa extracellular region and a 165 aa cytoplasmic tail. One potential soluble splice form is known where aa's 1056-1095 are deleted, eliminating the transmembrane region.
Catalog #N2028-50A
ApplicationsSuitable for use in ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 0.5-1ug/ml. The detection limit is ~0.3ng/well.
Western Blot: 0.1-0.2ug/ml. The detection limit is ~25ng/lane under non-reducing and reducing conditions.
Immunocytochemistry: 2-5ug/ml
Immunohistochemistry: 2-5ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, PBS. Aliquot and store at -20°C. Reconstituted product is stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycerol.
PurityPurified by human Nephrin affinity chromatography.
ImmunogenNS0-derived recombinant human Nephrin (aa23-1029) Accession # O60500
SpecificityRecognizes human Nephrin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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