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You are here:Home » Antibodies » Abs to Nephrin » Anti -Nephrin, phosphorylated (Tyr1217) (NPHN, CNF, Nephrosis 1 Congenital Finnish Type, NPHS1, Renal Glomerulus-specific Cell Adhesion Receptor)

Anti -Nephrin, phosphorylated (Tyr1217) (NPHN, CNF, Nephrosis 1 Congenital Finnish Type,
NPHS1, Renal Glomerulus-specific Cell Adhesion Receptor)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP
Nephrin (Renal glomenelus specific adhesion receptor) is a kidney glomerular filtration barrier protein that is an essential component of the interpodocyte-spanning slit diaphragm. Nephrin is located only in visceral glomerular epithelial cells, where it is targeted to intercellular junction of
mature podocyte foot processes (1). Mutations in the nephrin gene are associated with congenial nephritic syndrome (NPHS1) (2).
Catalog #N2028-50R
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:25,000-1:50,000
Immunoprecipitation: 1:20000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No9H483
HostRabbit
SourceMouse
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide and 0.05% BSA.
PuritySupernatant
ImmunogenA synthetic peptide corresponding to residues surrounding tyrosine 1217 of mouse Nephrin.
SpecificityRecognizes mouse Nephrin when phosphorylated at Tyr1217. Species Crossreactivity: Human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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