| Nephrin is encoded by NPHS1 gene (chromosome 19q13.1), the core protein of the interpodocytes slit diaphragm of kidney glomerulus, these podocytes are highly differentiated with characteristic interdigitating foot processes covering the outer glomerular basement membrane, the space between these foot processes is spanned by a tight junction to provide the large surface area for filtration. The mutated NPHS1 gene in congenital nephrotic syndrome of Finnish type, a human disease that leads to massive proteinuria in utero and nephrosis at birth. Nephrin is a transmembrane protein of immunoglobulin super family with 8 Ig like domains, identified in human (1241aa), rat (1252aa), and mouse (1256aa). |
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| Catalog # | N2028-62 |
| Applications | Suitable for use in ELISA, Western Blot. Other applications not tested. |
| Recommended Dilution | ELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml. See N2028-62A. |
| Western Blot (ECL): 1-10ug/ml. |
| Optimal dilutions to be determined by the researcher. |
| Control Peptide | N2028-62A: Nephrin Related Protein 1, Rat, Control Peptide (NEPH1) |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Rat |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, before the addition of glycerol to 40%. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | A synthetic 16aa peptide from rat NEPH1. Epitope: ~C-terminus, Cytoplasmic domain. |
| Specificity | Recognizes rat NEPH1. Species Sequence Homology: human, mouse - 100%. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
