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You are here:Home » Molecular Biology » Growth Factors-NGF » Nerve Growth Factor (NGF) (High Molecular Weight Complex)

Nerve Growth Factor (NGF) (High Molecular Weight Complex)

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Specifications

NGF is composed of 3 subunits; 1 beta subunit (2.5S), 2 alpha subunits and 2 gamma subunits.
Catalog #N2050-02
Biological AssayThe neurotrophic activity is in the range of 0.5-50ng/ml using rat PC12 cells cultured for 7-14 days. The recommended Concentration to be used in vitro for maintenance of sympathetic and immature sensory nerve cells is 50-100ng/ml medium.
Storage and StabilityLyophilized powder can be stored at -20ºC for 6 months. After reconstitution, store at -70ºC for 4-6 weeks. Avoid repeated freeze/thaw cycles to prevent loss of activity.
SourceMouse submaxillary gland
Purity~97% pure as determined by SDS-PAGE
ConcentrationAs reported
FormSupplied as a lyophilized powder. Reconstitute with sterile DMEM at a concentration of 10ug/mL and stored at-70°C. Medium with mNGF cannot be reused due to mNGF degradation.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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