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You are here:Home » Molecular Biology » Growth Factors-NGF » Nerve Growth Factor 2.5S, Mouse (Beta-Nerve Growth Factor, Beta-NGF, Ngf, Ngfb)

Nerve Growth Factor 2.5S, Mouse (Beta-Nerve Growth Factor, Beta-NGF, Ngf, Ngfb)

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Specifications

Mouse NGF (2.5S) was isolated from mouse submaxillary glands by method of Mobley et al (1976) and is a form of Beta-NGF that has identical biological properties. NGF is known to regulate the survival and development of certain sympathetic and sensory neurons. It is a dimer with 2 identical polypeptide chains and dimeric molecular weight of approximately 26,500 Da. Isolation and purification of NGF from mouse submaxillary glands yields preparations of NGF (2.5S) with identical biological activity but with cleavages at the amino terminus (with the loss of 8 amino acids) and/or at the carboxy-terminus (with the loss of arginine). These preparations are named 2.5 NGF (see reference below).
Catalog #N2050-05E
SourceIsolated from mouse submaxillary glands.
Biological. ActivityTested by stimulation of 8 day old chick embryo dorsal root ganglia.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: The homodimer has a molecular weight of ~26KD and the monomer ~13KD as confirmed by SDS PAGE.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceMouse submaxillary glands
Purity~90% (SDS electrophoresis)
ConcentrationAs reported
FormSupplied as lyophilized powder from 0.1M acetic acid under dry N2 with vacuum. Appears as near-white powder. Solution sterile filtered prior to drying.. Reconstitute with 25ul sterile PBS.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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