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Specifications

Catalog #	N2050-06
Dose for significant outgrowth of neurite using PC-12 rat pheochromocytoma cells	10ng/ml. 7S NGF has a variety of effects on sensory and sympathetic neuron growth and development.3 7S NGF is also needed for sympathetic nerve cells development and maintenance.
Biological Activity	Dose for significant outgrowth of neurite using PC-12 rat pheochromocytoma cells: 10ng/ml. 7S NGF has a variety of effects on sensory and sympathetic neuron growth and development. 7S NGF is also needed for sympathetic nerve cells development and maintenance.
Extinction Coefficient( E1%, 280nm, pH 7.0)	14.2
Sterility	Sterilized through a 0.2um membrane filter and packaged aseptically.
	NGF Assay:
	1. Coat 6 well dishes with rat tail collagen type I, at a concentration of 2.5-5.0ug/cm2. Incubate for one hour at 37°C. Aspirate and rinse 2 times with PBS before adding cell suspension.
	2. Prepare a cell suspension to yield 2x10e4 cells/ml in RPMI-1640/10% horse serum/ 5% FBS. Add 2mls of cell suspension to each well.
	3. Prepare NGF as described above under Rehydration to a final concentration of 1ug/ml. Add appropriate amounts of NGF to test at 0, 5, 10 and 20ng/ml final concentration.
	4. Incubate plates at 37°C for 3-5 days.
	5. Record neurite outgrowth at each NGF concentration.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight	130-140kD
Source	Male mouse submaxillary glands
Purity	Purified by gel filtration. Does not contain detectable amounts of EGF
Concentration	~1mg/ml
Form	Supplied as a liquid.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.