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You are here:Home » Molecular Biology » Growth Factors-NGF » Nerve Growth Factor, beta Recombinant, Human (NGFb)

Nerve Growth Factor, beta Recombinant, Human (NGFb)

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Specifications

Recombinant human nerve growth factor beta is a homodimer, glycosylated, polypeptide chain containing 2 identical 119aa and having a molecular mass of 16.9kD. The sequence of the first five N-terminal amino acids was found to be Ser-Ser-Ser-His-Pro.
Catalog #N2050-10B
Source
Human Nerve Growth Factor beta, from CHO cells.
Molecular Weight~16.9kD
Biological ActivityThe ED50, calculated by its ability to stimulate chick E9 DRG neurite outgrowth was found to be <1ng/ml, corresponding to a specific activity of >1x10e6Units/mg.
Endotoxin 0.1ng/ug
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute with sterile buffer or ddH2O, 0.1% HSA or BSA. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight16.9kD
SourceCHO Cells
Purity98% pure (HPLC, FPLC and reducing and non-reducing SDS-PAGE).
FormSupplied as a lyophilized powder from 30mM sodium acetate, pH 5.5, 1% HSA. Reconstitute with sterile deionized water to a concentration 100ug/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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