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Nerve Growth Factor, beta, Recombinant, Human (Beta-Nerve Growth Factor, Beta-NGF, Ngf, Ngfb, HSAN5, MGC161426, MGC161428)
Nerve Growth Factor, beta, Recombinant, Human (Beta-Nerve Growth Factor, Beta-NGF, Ngf,
Ngfb, HSAN5, MGC161426, MGC161428)
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| Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. | | | Catalog # | N2050-10H | | Source | DNA sequence encoding the human beta NGF protein sequence (containing the signal peptide, pro-peptide and the mature beta NGF sequence) was expressed in modified human 293 cells. | | Theoretical Peptide Sequence | YAEHKSHRGEYSVCDSESLWVTDKSSAIDIRGHQVTVLGEIKTGNSPVKQYFYETRCKEAR PVKNGCRGIDDKHWNSQCKTSQTYVRALTSENNKLVGWRWIRIDTSCVCALSRKIGRT | | Biological. Activity | ED50 of beta NGF is typically 0.2-1.0 ng/ml as measured in a cell proliferation assay using the human growth factor dependent TF-1 cell line. | | Applications | | | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: The NGF protein migrates at ~12-16kD in SDS-PAGE. It has a predicted molecular mass of 13.5kD. The protein separates into a number of isoforms with a pI between 9 and 10 in 2D PAGE due to post-translational modifications. The unmodified protein has a predicted pI of 9. Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Source | Modified human 293 cells | | Purity | ~95% (SDS-PAGE, silver stain) | | Concentration | As reported | | Form | Supplied as lyophilized powder from PBS, 1% HSA, 10% trehalose. Reconstitute with 500ul sterile PBS. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | Nerve Growth Factor (beta Polypeptide) |
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