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You are here:Home » Molecular Biology » Growth Factors-NGF » Nerve Growth Factor, beta, Recombinant, Human (Beta-nerve Growth Factor, Beta-NGF, NGFb, NGF, HSAN5, MGC161426, MGC161428)

Nerve Growth Factor, beta, Recombinant, Human (Beta-nerve Growth Factor, Beta-NGF, NGFb,
NGF, HSAN5, MGC161426, MGC161428)

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Specifications

Catalog #N2050-10K
SourceRecombinant corresponding to 119aa of human beta-Nerve Growth Factor expressed in E.coli is a homodimer, glycosylated, polypeptide chain.
Molecular Weight~17kD
Biological ActivityThe ED50,calculated by its ability to stimulate chick E9 DRG neurite outgrowth was found to be 1x106 units/mg.
Dimers and Aggregates 1% (SDS-PAGE)
Protein ContentProtein quantitation was carried out by two independent methods:1.UV spectroscopy at 280nm using the absorbency value of 1.1945 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Recombinant corresponding to beta-Nerve Growth Factor as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~17kD
Purity~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug)
ConcentrationNot determined
FormSupplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesNerve Growth Factor (beta Polypeptide)


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