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You are here:Home » Antibodies » Abs to Neurofilament » Anti -Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes)

Anti -Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Nestin is a marker of multipotent neuroectodermal cells and is downregulated as the cells cease division and differentiate to their neural or glial lineages. Expression of Nestin is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.
Catalog #N2060-04A
ApplicationsSuitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionImmunohistochemistry (paraffin): 5ug/ml
ELISA: 1:20,000-1:80,000
Western Blot: 1:500-1:3,000
Requires antigen retrieval using heat treatment for 20 minutes prior to staining of paraffin sections.
Optimal dilutions to be determined by the researcher.
Histology Positive Control TissueHuman kidney
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant human Nestin (aa1484-1500).
SpecificityRecognizes human Nestin. Species crossreactivity: macaque, mouse, bovine, canine, hamster, rat, rabbit.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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