| Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor |
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| Catalog # | N2060-04E |
| Applications | Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested. |
| Recommended Dilution | Immunohistochemistry: 1:10,000-1:30,000 |
| Western Blot: 1:20,000-1:50,000 |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1906 IgY, Chicken Pab Rb x Ch |
| I1906-10 IgY, Chicken (HRP) Rb x Ch |
| I1906-12 IgY, Chicken (FITC) Rb x Ch |
| I1906-14 IgY, Chicken (AP) Rb x Ch |
| Positive Control | Mouse brain |
| Marker | Oligodendrocyte Marker |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgY |
| Host | Chicken |
| Source | Mouse |
| Concentration | ~0.25mg/ml |
| Form | Supplied as a liquid in 10mM PBS pH 7.2, 0.02% sodium azide. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Three different affinity-purified anti-peptide antibodies were combined to make this product. Cellular localization: cytoplasmic. |
| Specificity | Recognizes mouse nestin. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
