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You are here:Home » Antibodies » Abs to Neurofilament » Anti -Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes)

Anti -Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes)

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Specifications

Clone Host Grade Applications
Polyclonal Chicken Affinity Purified B IH
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor
Catalog #N2060-04E
ApplicationsSuitable for use in Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionImmunohistochemistry: 1:10,000-1:30,000
Western Blot: 1:20,000-1:50,000
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1906 IgY, Chicken Pab Rb x Ch
I1906-10 IgY, Chicken (HRP) Rb x Ch
I1906-12 IgY, Chicken (FITC) Rb x Ch
I1906-14 IgY, Chicken (AP) Rb x Ch
Positive ControlMouse brain
MarkerOligodendrocyte Marker
Storage and StabilityMay be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgY
HostChicken
SourceMouse
Concentration~0.25mg/ml
FormSupplied as a liquid in 10mM PBS pH 7.2, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenThree different affinity-purified anti-peptide antibodies were combined to make this product. Cellular localization: cytoplasmic.
SpecificityRecognizes mouse nestin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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