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You are here:Home » Antibodies » Abs to Neurofilament » Anti -Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes)

Anti -Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IC IF
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.
Catalog #N2060-04H
ApplicationsSuitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionWesterm Blot: 1:1000-1:5000. Doublet band seen ~220-240kD.
Immnohistochemistry: Paraffin and Frozen
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No9A19
HostMouse
SourceHuman
Concentration~2mg/ml
FormSupplied as a liquid.
PurityPurified by Protein G affinity chromatography.
ImmunogenA fragment of the human nestin protein corresponding to residues 1464-1614.
SpecificityRecognizes human nestin. It does not crossreact with rodent protein.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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