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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IH FC IF

Nestin is a class VI intermediate filament protein expressed predominantly in stem cells of the neural tube but absent from virtually all differentiated CNS cells. In the CNS, nestin is downregulated upon differentiation and replaced by neurofilaments. Transient expression of nestin has been postulated as a key step committing cells to the neural differentiation pathway. Nestin expression has also been observed in pancreatic hematopoietic stem cell populations.
Catalog #	N2060-04L
Applications	Suitable for use in ELISA, Western Blot, Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilutions	ELISA: 1:1000
	Western Blot: 1:100-1:500
	Flow Cytometry: 1:10-1:50
	Immunohistochemistry: 1:50-1:100
	Immunofluorescence: 1:200
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~0.25mg/ml
Form	Supplied as a liquid in PBS, 0.09% sodium azide.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Synthetic peptide corresponding to aa1395-1425 surrounding S1409 of human NES, conjugated to KLH.
Specificity	Recognizes human NES.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.