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You are here:Home » Antibodies » Antibodies-Neurofilament » Anti -NES (S1409) (Nestin, Nbla00170) (Biotin)

Anti -NES (S1409) (Nestin, Nbla00170) (Biotin)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH FC IF
Nestin is a class VI intermediate filament protein expressed predominantly in stem cells of the neural tube but absent from virtually all differentiated CNS cells. In the CNS, nestin is downregulated upon differentiation and replaced by neurofilaments. Transient expression of nestin has been postulated as a key step committing cells to the neural differentiation pathway. Nestin expression has also been observed in pancreatic hematopoietic stem cell populations.
Catalog #N2060-04L-Biotin
ApplicationsSuitable for use in ELISA, Western Blot, Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended DilutionsELISA: 1:1000
Western Blot: 1:100-1:500
Flow Cytometry: 1:10-1:50
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:200
Optimal dilutions to be determined by the researcher.
Storage and StabilityStore product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note Applications are based on unconjugated antibody.
Clone TypePolyclonal
ConcentrationAs Reported
FormSupplied as a liquid in PBS, pH 7.2. No preservative added.
PurityPurified by Protein A affinity chromatography.
ImmunogenKLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1389-1416 of human NES, UniProt Accession #P48681.
SpecificityRecognizes human NES.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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