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You are here:Home » Antibodies » Abs to Neurofilament » Anti -NES (S1409) (Nestin, Nbla00170) (PE)

Anti -NES (S1409) (Nestin, Nbla00170) (PE)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH FC IF
Nestin is a class VI intermediate filament protein expressed predominantly in stem cells of the neural tube but absent from virtually all differentiated CNS cells. In the CNS, nestin is downregulated upon differentiation and replaced by neurofilaments. Transient expression of nestin has been postulated as a key step committing cells to the neural differentiation pathway. Nestin expression has also been observed in pancreatic hematopoietic stem cell populations.
Catalog #N2060-04L-PE
ApplicationsSuitable for use in FLISA, Western Blot, Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended DilutionsFLISA: 1:1000
Western Blot: 1:100-1:500
Flow Cytometry: 1:10-1:50
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:200
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze R-Phycoerythrin conjugates. Light sensitive. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to R-Phycoerythrin (R-PE).
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to a portion within aa1395-1425 surrounding S1409 of human NES, conjugated to KLH.
SpecificityRecognizes human NES.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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