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Nestin, Mouse, Rat, Primer Pair (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes)
Nestin, Mouse, Rat, Primer Pair (ESTM46, FLJ21841, Intermediate Filament Protein,
Nbla00170, Nes)
Pricing
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| Mouse/rat nestin specific PCR primers for RT-PCR (reverse transcription followed by polymerase chain reaction) analysis of mRNA expression. | | | Catalog # | N2060-04P | | Components | Primer Pair: Lyophilized. Each vial contains 375 pmoles of each primer. Adjust to a final concentration of 7.5 pmoles/ul by resuspending Primer Pair in either 50ul autoclaved deionized water or 0.1X TE buffer (1mM Tris HCl, pH8.0 @ 25°C; 0.1mM EDTA, pH 8.0 @ 25°C). | | Positive Control 24 | Lyophilized. Each vial contains 150 ng synthetic double-stranded DNA. Resuspend in 30ul autoclaved deionized water or TE buffer. To prevent contaminating samples and reagents, it is strongly recommended that the Positive Control is resuspended in a separate location from where PCR reactions are set up. Use a different pipette than one used for PCR set up. The Positive Control is not intended for quantitative purposes. | | GenBank Accession Number | NM_016701 (mouse) and NM_012987 (rat) | | Product Sizes | cDNA: 388 base pairs (bp) | | Genomic DNA: 3620 bp product predicted. No product observed. | | Positive Control 24: 275 bp | | Alternate splice variants: None reported. | | Suggested Reaction Conditions | For each 50ul Polymerase Chain Reaction mixture, 2ul of the primer pair stock solution should be used. Sufficient reagent is supplied for 25 separate amplification reactions of the gene fragment. | | Thermocycling Conditions | A typical 50ul PCR would involve a 4 minute denaturation step at 94ºC, followed by 30-35 cycles of 45 seconds at 94ºC, 45 seconds at 55ºC and 45 seconds at 72ºC, with a final 10 minute extension step at 72ºC. | | Storage and Stability | The Primer Pair resuspended in water or TE buffer is stable for up to one year at -20°C in a non-frost free freezer. Aliquot in single use portions. Do not use past the expiration date above. Avoid repeated freeze-thaw cycles. | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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