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Anti -Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes)
Anti -Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
IC FC |
|
| Nestin is a class VI intermediate filament protein that is expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells. Its transient expression is a critical step in the neural differentiation pathway. Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors and hematopoietic progenitors. | | | Catalog # | N2060-05A | | Applications | Suitable for use in Flow Cytometry and Immunocytochemistry. Other applications not tested. | | Recommended Dilutions | Flow Cytometry: 2.5ug labels 10e6 cells for intracellular staining. Validated using A172 human glioblastoma cell line fixed with parafomaldehyde and permeabilized with saponin. | | Immunocytochemistry: 8-25ug/ml. Validated using immersion fixed human neural progenitor cells. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 3k1 | | Host | Mouse | | Source | Human | | Concentration | ~0.5mg/ml (after reconstitution) | | Form | Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | NSO cells transfected with aa618-1618 of human Nestin. | | Specificity | Recognizes human Nestin. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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