| Nestin is a class IV intermediate filament protein abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. This cytoskeletal protein has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. |
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| Also, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. These highly specific antibodies to human nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. |
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| Catalog # | N2060-05B |
| Applications | Suitable for use in Immunocytochemistry, Immunohistochemistry, ELISA and Western Blot. |
| Other applications not tested. |
| Recommended Dilution | Immunocytochemistry: 1:100-1:200. Using a fluorescein or rhodamine conjugated anti-mouse secondary antibody. |
| Immunohistochemistry (frozen/paraffin): 1:100-1:200. Using a fluorescein or rhodamine conjugated anti-mouse secondary antibody. |
| Western Blot: 1:1000-1:5000 using chemiluminescent detection. |
| Optimal dilutions to be determined by the researcher. |
| Hybridoma | Nestin transfected NS0 mouse myeloma cells. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 5C93 |
| Host | Mouse |
| Source | Human |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, before the addition of glycerol to 40%. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | 150-amino-acid fragment from cloned human nestin conjugated to glutathione S-transferase |
| Specificity | Reacts exclusively with the human nestin protein. |
| Species Crossreactivity | Does not crossreact with rodent Nestin. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
