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You are here:Home » Antibodies » Abs to Neurofilament » Anti -Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes)

Anti -Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH IC FC
Nestin is a class VI intermediate filament protein that is expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells. Its transient expression is a critical step in the neural differentiation pathway. Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors and hematopoietic progenitors.
Catalog #N2060-05D
ApplicationsSuitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000
Immunohistochemistry (Formalin-fixed, paraffin-embedded): 20ug/ml
Immunocytochemistry: 1:100-1:500
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2C1.3A11
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS, 0.1% sodium azide.
PurityPurified by Protein G affinity chromatography.
Immunogen150aa fragment from the cloned human Nestin (GST).
SpecificityRecognizes human NESTIN.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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