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Anti -Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes)
Anti -Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B IP IH IC FC |
|
| Nestin is a class VI intermediate filament protein that is expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells. Its transient expression is a critical step in the neural differentiation pathway. Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors and hematopoietic progenitors. | | | Catalog # | N2060-05D | | Applications | Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000-1:5000 | | Immunohistochemistry (Formalin-fixed, paraffin-embedded): 20ug/ml | | Immunocytochemistry: 1:100-1:500 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 2C1.3A11 | | Host | Mouse | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid in PBS, 0.1% sodium azide. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | 150aa fragment from the cloned human Nestin (GST). | | Specificity | Recognizes human NESTIN. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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